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KMID : 0613819990090010099
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Journal of Life Science
1999 Volume.9 No. 1 p.99 ~ p.105
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Discovery of Antioxidant on Human Low Density Lipoprotein(LDL) by Bacillus sp. RH-5 Isolated from Marine Origin
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Abstract
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The aim of this studies were carried out to investigate the antioxidant on low density lipoprotein(LDL) using substances extracted from Bacillus sp. RH-5. The antioxidantive substances produced extracellula in the culture broth by Bacillus sp. RH-5 was obtained by elution of chloroform : methanol from silicagel column (80 cm \times 100 cm) chromatography. Band 4 eluted from fraction 3 by TLC was appeared at highest level of antioxidative activity using thiocyanate method. Band 4 at a concentration of 100 or 200 \mu g/ml inhibited oxidation of LDL induced by the mouse transformed macrophage. According to IR. NMR or GC/MASS, the antioxidant substance was identified as 5-hydroxyindole
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